RISK FACTOR OF DEGREE OF AGGLUTINATION IN ROSE BENGAL TEST: STUDY IN DAIRY FARMER SUSPECTED BRUCELLOSIS IN SLEMAN, DAERAH ISTIMEWA YOGYAKARTA

Abstract

The incidence of brucellosis in humans in Indonesia has not been reported as well as zoonotic disease. It made most people do not know if brucellosis can be transmitted to humans. On March 2015, there was reported three farmers at one dairy farm got brucellosis. So it needed deep and specific research. This study aims to determine the prevalence and factors associated with suspected brucellosis, based on the degree of agglutination of dairy farmers. The population was all dairy farmer in Cangkringan. This study was conducted by cross sectional design; individual interviewed using a questionnaire containing questions about disease Brucellosis. Blood samples were tested using Rose Bengal Test (RBT) in laboratorium. 249 people joined by randomly sampling in 650 farmers. Data was proceed by Stata using Spearman's test to look at the correlation of each factor. There were two positive serological Brucellosis (0,8%). Spearman’s correlation analysis between degrees of agglutination of RBT with risk factor showed using of protective equipment (Ï=-0,0243 P=0,7024); using of disinfectants (Ï=-0,0896 P=0,1586); time of breed (Ï=0,0100 P=0,8758); cattle vaccination status (Ï= -0,0273 P=0,6680) were not associated with degree of agglutination. Have a history of brucellosis in humans (Ï=0,4399 P=0,0000); direct contact with the infected cow (Ï=0,8149 P=0,0000); history of infected cattle (Ï= 0,5727 P=0,000) were associated with degree of agglutination. The prevalence of brucellosis incidence on dairy farmers was 0,8%. There was a strong relationship between having a history of brucellosis in humans, direct contact with infected animals, history of animals infected with the degree of agglutination at RBT on dairy farmers.

Keywords: Brucellosis, factors, risks, agglutination, cows, humans