Mengungkap Kandungan Tanin Ekstrak Buah Mengkudu (Morinda Citrifolia L) Menggunakan Metode Microwave Assisted Extraction (MAE): Pilot Study Bahan Herbal
Abstract
Tanin merupakan senyawa fenolik yang paling umum ditemukan dalam banyak tumbuhan,
termasuk dalam buah mengkudu. Tanin mempunyai manfaat penting dalam kebutuhan farmasi,
pangan, dan herbal. Metode ekstraksi yang efektif dan cepat untuk mendapatkan senyawa
bioaktif dari bahan tanaman adalah metode ekstraksi yang dibantu oleh microwave. Penelitian
ini bertujuan untuk mengidentifikasi senyawa tanin dalam buah mengkudu dengan metode
ekstraksi menggunakan metode Microwave Assisted Extraction (MAE). Penelitian ini adalah
eksperimen di laboratorium dengan menggunakan sampel buah mengkudu. Buah mengkudu
dikeringkan kemudian dijadikan serbuk simplisia. Ekstraksi menggunakan pelarut etanol 96%
dan alkohol 70%, kemudian dipekatkan dengan metode MAE dengan waktu 2 menit dengan suhu
50 derajat C. Ekstrak mengkudu kemudian diidentifikasi tanin dengan menggunakan pelarut FeCl3.
Hasil penelitian menunjukan bahwa ekstrak alkohol 70% buah mengkudu mengandung senyawa tanin.
Keywords
Full Text:
PDFReferences
De Araujo AA, Soares LAL, Assunçao Ferreira MR, De Souza Neto MA, Da Silva GR, De Araujo RF, et al. Quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone-water extracts of Libidibia ferrea,Parapiptadenia rigida and Psidium guajava. J Ethnopharmacol [Internet]. 2014;156:88–96.Available from: http://dx.doi.org/10.1016/j.jep.2014.07.031
Almeida ES, de Oliveira D, Hotza D. Properties and Applications of Morinda citrifolia(Noni): A Review. Comprehensive Reviews in Food Science and Food Safety. 2019.
Barraza-Elenes C, Camacho-Hernandez IL, Yahia EM, Zazueta-Morales JJ, Aguilar-Palazuelos E, Heredia JB, et al. Analysis by UPLC-DAD-ESI-MS of phenolic compounds and HPLC-DAD-based determination of carotenoids in Noni (Morinda citrifolia L.) Bagasse.J Agric Food Chem. 2019;67(26):7365–77.
Wilson P, Rojan P, Kumar P, Sabu T. Tannin Acyl Hydrolase Production by Citrobacter sp.isolated from Tannin rich Environment, using Tamarindus indica seed powder. J Appl Sci Environ Manag. 2010;13(4).
Huang HL, Liu CT, Chou MC, Ko CH, Wang CK. Noni (Morinda citrifolia L.) fruit extracts improve colon microflora and exert anti-inflammatory activities in caco-2 cells. J Med Food.2015;18(6):663–76.
Huang HL, Ko CH, Yan YY, Wang CK. Antiadhesion and anti-inflammation effects of noni (Morinda citrifolia) fruit extracts on AGS cells during Helicobacter pylori infection. J Agric Food Chem. 2014;62(11):2374–83.
Buanasari B, Dhamayanti SMS, Suryaningsih S. Effect of Variation Conditions of The Extraction Process of Morinda Citrifolia L Leaves Using Ultrasound-Assisted Extraction Method (Uae). J Sci Technol Res Pharm. 2021;1(1):25–33.
Gotama B, Rahman AK, Ahmad A, Hariyadi A. Extraction of rice bran oil using microwave-assisted extraction and green solvents. IOP Conf Ser Earth Environ Sci. 2022;1105(1).
Li Y, Li S, Lin S-J, Zhang J-J, Zhao C-N, Li H-B. Microwave-Assisted Extraction of Natural Antioxidants from the Exotic Gordonia axillaris Fruit: Optimization and Identification of Phenolic Compounds. Molecules [Internet]. 2017 Sep 6;22(9). Available from:http://www.ncbi.nlm.nih.gov/pubmed/28878178
Juodeikaite D, Zilius M, Briedis V. Preparation of Aqueous Propolis Extracts Applying Microwave-Assisted Extraction. Processes. 2022;10(7).
Kaderides K, Papaoikonomou L, Serafim M, Goula AM. Microwave-assisted extraction of phenolics from pomegranate peels: Optimization, kinetics, and comparison with ultrasounds extraction. Chem Eng Process - Process Intensif [Internet]. 2019;137:1–11. Available from: https://doi.org/10.1016/j.cep.2019.01.006
Hu Q, He Y, Wang F, Wu J, Ci Z, Chen L, et al. Microwave technology: a novel approach to the transformation of natural metabolites. Chinese Med (United Kingdom). 2021;16(1):1–22.
Izza N, Sumarni N, Pramesi ND, Mahardi UA, Dewi SR. Extraction of Phenolic Compounds from Coleus amboinicus Leaves by Microwave-assisted Extraction: Optimization of the Operating Condition. Int J Adv Sci Eng Inf Technol. 2022;12(3):946–52.
Ky I, Teissedre PL. Characterisation of Mediterranean grape pomace seed and skin extracts: Polyphenolic content and antioxidant activity. Molecules. 2015;
Kocabey N, Yilmaztekin M, Hayaloglu AA. Effect of maceration duration on physicochemical characteristics, organic acid, phenolic compounds and antioxidant activity of red wine from Vitis vinifera L. Karaoglan. J Food Sci Technol. 2016;
Liao J Le, Guo J, Niu YH, Fang T, Wang FZ, Fan YL. Flavonoids from Lycium barbarum leaves attenuate obesity through modulating glycolipid levels, oxidative stress, and gut bacterial composition in high-fat diet-fed mice. Front Nutr. 2022;9(July):1–14.
Sharma K, Pachauri SD, Khandelwal K, Ahmad H, Arya A, Biala P, et al. Anticancer Effects of Extracts from the Fruit of Morinda Citrifolia (Noni) in Breast Cancer Cell Lines. Drug Res (Stuttg). 2015;
Abstract View: 21, 
PDF Download: 74
Refbacks
- There are currently no refbacks.
Prosiding Seminar Nasional
Universitas Respati Yogyakarta
Fax : 0274-489780
This work is licensed under a Creative Commons Attribution 4.0 International License.